- Gene Editing
- Single Cell
4titude® Ltd specialises in the design, manufacture and marketing of consumables and bench top instrumentation for the life sciences industry.
Since our foundation in 2005, we continue to grow a range of innovative products, focusing on:
- PCR, QPCR & sequencing tubes, plates and seals for manual and robotic use
- Assay Plates and seals for imaging, recommended by plate reader manufacturers
- Quality polypropylene storage plates of varying volumes for manual and robotic use
- Specific adhesive plates seals, including gas permeable and moisture resistant seals
- Automated and semi-automated heat sealing instrumentation and materials
- Liquid handling robotics
AddressThe North Barn
Surry Hills Business Park
Polypropylene (PP) is the best plastic material for PCR tubes as it is chemically inert, resistant to solvents, and well suited for injection moulding - allowing for production of thin-walled tubes for optimum PCR results. DNA has been shown to bind to PP tubes especially at high ionic strength, despite the very hydrophobic nature of this material. Different PP polymers are used for the production of PCR consumables and as they differ in their characteristics including surface charges, they consequently bind DNA in varying amounts. DNA binding to PP surfaces has typically only been an issue for reaction tubes and storage vessels but not for PCR/qPCR tubes. DNA sticking to tube walls is either released during denaturation steps and/or remains accessible for amplification. Nevertheless, due to a progressing volume miniaturisation and with new technologies such as Next Generation Sequencing (NGS), PCR/qPCR tubes are more and more also recommended for other applications which may require ultra-low DNA binding.
4titude have extensive experience and superior technology for making PCR plates with ultra-thin polypropylene wells fitted into a robust polycarbonate frame that provides excellent stability. Our Random Access plates are an extension of this technology to supply a novel 96 well plate with individually sealable and removable wells combining both flexibility and robustness. Read more about our PCR plate range. Random Access (RA) plates provide the greatest flexibility in PCR plates, affording handling of individual wells in a 96 well plate format. Each well is moulded into a rigid frame suitable for use with automation. Once containing your sample or reagents, plates can be sealed using random access heat seals resulting in filled wells which can be removed from the plate as required. The wells are made from medical grade polypropylene which is perfectly suited for use in PCR as well as for long term storage. The exact number of wells required can be used, meaning no wastage of consumables or reagents. Each well clicks into place within the frame, holding it securely for use with automation and for transport. Additionally, the fit of the tubes is not compromised following a PCR run due to resistance of the rigid frame to thermal expansion.
Heat sealing is the gold standard method of plate and tube sealing. It prevents sample loss and maximises sample security, by facilitating a 100% complete seal and preventing evaporation, leakage and contamination. The sealing performance of heat seals is superior to all other methods including cap, mat and adhesive sealing. The variability of sealing consistency seen when using adhesive seals, caps or mats is completely removed. The optimized sealing performance of a heat seal allows the use of smaller reagent volumes, leading to reagent cost savings such that heat sealing is the most cost efficient sealing method for a wide range of applications.
4titude® offers the widest range of adhesive sealing solutions available on the market. You have the option to choose your seal based on a wide variety of properties offered including: peelability, pierceability, gas permeability, optical clarity, temperature stability and solvent resistance. Most of our adhesive sheet seals are supplied with convenient tabs on both ends for ease of application. These tabs enable easy peeling for sealing removal without leaving adhesive residue on the sealing surface. We also offer a number of our seals in various roll formats compatible with most roll sealers on the market, for ease of use or for automation. To obtain the best sealing results with adhesive seals we strongly recommend the use of high quality plates, such as 4titude® plates, with superior flatness for optimal seal application. PCR and qPCR plates should have raised well rims to facilitate effective sealing and prevent well to well contamination (all 4titude® PCR plates have raised rims).
The 4s3™ is a semi-automatic sheet heat sealer which is compatible with a wide range of seals and plates of differing designs and heights. With variable temperature and time settings, sealing conditions are easily optimised to produce a 100% seal, eliminating sample loss. Plate and seal are placed on the holder, the "Operate" button pressed and the drawer automatically closes. The sealing process is controlled by an electric mechanism. This unique feature eliminates operator variation, giving a consistent seal every time and reducing the damage caused to microplates by over-sealing.
The main advantage of the a4S is that it does not require an air supply, which gives it the flexibility to be used on standard lab benches as a stand-alone unit, or integrated within a robotic set‑up. The sealer’s rapid heating element enables a fast start-up, and the stand-by function saves energy when the unit is not in use. The a4S sealer is compatible with a wide range of SBS footprint plates, including all PCR plate formats from 96 well to 1536 well, assay plates, deep well storage plates and microplates. Varying plate heights, from 3 mm to 60 mm can be accommodated with minimal adjustments of the instrument. Plates are presented in portrait format and the plate position on the shuttle allows good access for robotic integration.
Designed to directly print text, linear barcodes, 2D codes and graphics on plastic laboratory tubes using patent pending Thermal Pixel Printing technology Prints directly on the surface of tubes from 0.2 ml to 50 ml - Labels or stickers are not required Clearer and more durable marking compared to marker pen - Consistently legible text and barcodes at high resolutions Resistant to alcohol, water, liquid nitrogen, DMSO and mechanical abrasion - Long lasting sample identification at competitive “per mark” cost
4titude's® 4LAB™ is an automated, high-precision pipetting system specifically designed for low-volume liquid handling. For acceptable PCR results, small volumes of reagents need to be dispensed accurately and consistently, which is tedious, time consuming and prone to human error. It is available as a 4-position and a 6-position workstation. 4LAB™ is designed to replace manual PCR/qPCR sample preparation. Accuracy, precision and consistency are guaranteed, and reagent wastage is reduced. Unlike complex, multi-purpose robotic systems, our instrument was designed for the researcher without prior robotic experience.
At 4titude®, we offer a wide range of PCR consumables for low to medium throughput applications. Our standard one-piece PCR plates are manufactured from virgin polypropylene in our Class 7 ISO certified cleanroom production facility, and comply to the same stringent QC requirements as our FrameStar® range. The ultra-thin walled tubes of our standard PCR plates maximise heat transfer and the raised rims facilitate sealing. Our range includes non-skirted, semi-skirted, and fully skirted plates, available in clear or white (for qPCR techniques), with additional colours for non-skirted plates available. All our PCR consumables are certified free from RNase, DNase, and human genomic DNA. These plates fit most thermal cyclers.
Our FrameStar® 2-component technology reduces evaporation from PCR plates, improving your results and allowing you to reduce the volume of expensive reagents to save you money. FrameStar® plates combine the advantages of ultra-thin walled polypropylene tubes for optimum PCR results, with a rigid polycarbonate frame to increase the thermal stability of the plate during the PCR process. What results is a plate that not only provides you with highly accurate results, but also allows you to use less reagent in the process, as the polycarbonate frame does not warp and expand during rapid temperature change. This means the integrity of the seal is not compromised, preventing sample evaporation.